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Proficiency in Microbial
Biotechnology
1. Isolation of Micro-organisms from soil by serial dilution
2. Pure culture prepation
3. Identification of organisms by
a. Staining techniques
i. Grams staining
ii. Flagelar staining
iii. Endospore staining
iv. Capsular staining
b. Biochemical tests
i. IMVIC test
ii. Caesein hydrolysis
iii. Starch hydrolysis
iv. Gelatin liquification
v. Catalase test
vi. TSI test
4. Growth curve
5. Mutation studies
6. Bacterial conjugation
Duration : 15 Days
Eligibility :M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, P.hd., B.Tech. B.E. (Chemical / Biotech)
Proficiency in Recombinat DNA Technology
1. Isolation of plant DNA
2. Purification of plant DNA
3. Isolation of animal DNA
4. Isolation of Plasmid DNA
5. Isolation of genomic DNA from prokaryotes
6. Quantitative and Qualitative analysis of DNA
7. Agarose Gel Electrophoresis
8. Elusion of inserts from the Gel
9. Restriction Digestion
10. Ligation
11. Transformation
12. Gene cloning and expretion
13. Southern Blotting
14. Western Blotting
Proficiency in Enzymology
1. Isolation of micro-organisms from soil by serial
dilution
2. Pure culture preparation
3. Screening for the enzyme production
4. Batch Culture
a. Solid state fermentation
b. Submerged fermentation
5. Enzyme purification
a. Salt precipitation technique
b. Dialysis
6. Ion Exchange Chromatography
7. Enzyme assay
8. Quantitative estimation of Protein
9. Enzyme kinetic
a. pH
b. Temperature
c. Substrate conc
d. Activator
e. Inhibitor
10. SDS - PAGE
11. Immobilization of Enzymes
Duration : 15 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, B.Tech. B.E. (Chemical / Biotech)
Proficiency in Immunotechnology
1. Isolation & Purification of Ig G from blood sample
a. Salt Precipitation
b. Dialysis
c. Ion exchange Chromatography
d. Quantitative estimation of proteins
e. SDS PAGE
2. ODD test
3. RID test
4. Immuno electrophoresis
5. HCG test (Pregnancy test)
6. Blood Grouping and Rh typing
7. ELISA
8. Widal
9. RPR test
Duration : 15 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, B.Tech. B.E. (Chemical / Biotech)
Proficiency in Plant Tissue Culture
1. Instrumentation in Plant tissue culture
i. Hot air oven
ii. Autoclave unit
iii. Culture vessels and racks
iv. Incubation chamber
v. Rotary Shaker
vi. Deep freezer
vii. Laminar air flow unit
2. Sterilization technique:
i. Glassware
ii. Explant preparation
3. Preparation of culture media:
i. Preparation of stock solutions of MS, B5, Nitsch and LS media
ii. Preparation of hormone stocks solution.
iii. Preparation of MS, Gamborgs, Nitsch and LS media
4. Callus culture:
i. Initiation and maintenance of callus from the inter nodal explants of
stem
explants of any available plant material
ii. Embryo culture
iii. Anther culture
iv. Meristam culture
5. Techniques involved in Agrobacterium mediated gene
transformation
i. Leaf Disc Preparation
ii. Co-cultivation
iii. Selection media transfer
6. Encapsulation of embryo
Duration : 15 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, B.Tech. B.E. (Chemical / Biotech)
Proficiency in Industrial Microbiology
1. Sterility Techniques
2. Isolation of Micro-organisms from natural sources
3. Staining Technique
i. Grams staining
ii. Endospore staining
4. Biochemical Tests
i. Gelatin liquification
ii. Catalase test
iii. TSI test
5. Bacterial Growth Curve
6. Mutation Studies
7. Screening of Micro Organisms for Industrial applications
i. Caesein hydrolysis
ii. Starch hydrolysis
iii. Cellulose hydrolysis
9. Antibiotic assays
10. Quality control of milk
11. Water analysis
Duration : 15 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, B.Tech. B.E. (Chemical / Biotech)
Proficiency in Clinical Microbiology
1. Isolation of Micro-organisms from clinical sample
2. Grams staining
3. IMVIC test
4. Starch hydrolysis
5. Gelatin liquification
6. H2S production test
7. ELISA test
8. Widal test
9. Detection of blood group and Rh factor
10. Catalase test
11. Pregnancy test
12. Detection of urinary protein
13. Detection of urinary glucose
14. Detection of ketone bodies in urine sample
15. Heamoglobulin test
16. RPR test
17. WBC count
Duration : 15 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, B.Tech. B.E. (Chemical / Biotech) |
