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INTRODUCTION
:Biotechnology is a multidisciplinary science comprising
various facets of Biology. This course provides a advanced
theoreticaland practical aspects required for a Biotech
industry.
MODULE I - Microbial Biotechnology
1. Fundamentals in Microbiology
2. Sterilization Techniques
3. Microbiological culture media and its importance in
Biotechnology
4. Isolation of Micro-organisms from air and soil
5. Pure culture preparation
6. Identification of organisms by
a. Staining techniques
b. Biochemical tests
7. Growth curve
Applied Techniques
1. Antibiotic assays
2. Mutational studies by Replica plating method
3. Bacterial conjugation
4. Screening for Enzymes
5. Antogonism studies
6. Isolation of protozoa from samples
7. Isolation & Identification of fungi
8. Production of secondary metabolites (Acids, Wine,
Enzymes, Antibiotics)
MODULE II - Recombinant DNA Technology
I. Fundamentals
1. Isolation of plant DNA SDS method,C-TAB method
2. Isolation of RNA
3. Isolation of animal DNA
4. Isolation of Plasmid DNA
5. Isolation of genomic DNA from prokaryotes
6. Quantitative and Qualitative analysis of DNA
II. Applied Techniques
1. Tm of DNA-Melting point of DNA
2. Agarose Gel Electrophoresis
3. Elusion of inserts from the Gel
4. Restriction Digestion
5. Ligation
6. Transformation
7. Gene cloning and expression
8. Southern Blotting
9. Western Blotting
10. PCR Techniques
MODULE III - Fermentation and downstream Processing
I. Fundamentals
1. Fermentation Fundamental
2. Submerged fermentation
3. Solid state fermentation
4. Fermentation systems
a. Conical flask
b. Vessels
c. Fermentors
II. Applied aspects depends on the desired products
(Citric acid/Amino acids/ Enzymes/Antibiotics)
1. Down stream processing
a. Extra cellular
b. Intra cellular
2. Cell separation techniques
a. Centrifugation
b. Membrane filtration
c. Cell lysing
3. Isolation /Purification of desire products
a. Precipitation techniques
b. Solvent extraction
c. Dialysis
d. Ion exchange chromatography
e. Distillation
III Analysis of Metabolites/Products
1. Thin layer chromatography
2. Melting point determination
3. Spectrophotometer determination
4. Titrometric
5. Electrophoresis
MADULE IV - Immunological Techniques
I. Agglutination and Precipitation Methods
1. Blood grouping and Rh typing
2. Fibril Agglutination test(Widal test)
3. VDRL Test/ RPR
4. Hbs Ag Test
II. Gel diffusion Methods
1. Ouchterlony Double Diffusion
2. Single Radial Immunodiffusion
3. Immunoelectrophoresis
4. Counter current immunoelectrophoresis
5. Rocket immunoelectrophoresis |
