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Diploma in Microbial
Biotechnology
INTRODUCTION :Micro-organisms are biological
factories, which provide majority of the biotechnological
products so far commercialized. With the advent of
recombinant DNA technology, the scope and importance of
microorganisms and its in depth study has assumed tremendous
importance. This course provides detailed enlightenment into
the practical world of microbial Biotechnology.
Fundamentals
1. Fundamentals in Microbiology
2. Sterilization Techniques
3. Microbiological culture media and its importance in
Biotechnology
4. Isolation of Micro-organisms from air and soil
5. Pure culture preparation
6. Identification of organisms by
a) Staining techniques
b) Biochemical tests
7. Growth curve
Applied Techniques
1. Antibiotic assays
2. Mutational studies by Replica plating method
3. Bacterial conjugation
4. Screening for Enzymes
5. Antogonism studies
6. Isolation of protozoa from samples
7. Isolation Identification fungi
8. Production of secondary metabolites
(Acids, /Wine,/ Enzymes,/Antibiotics)
Duration : 30 days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.E. (Biotek / Chemical)
Diploma In Recombinant DNA &
Genetic Engineering
INTRODUCTION :RDNA and
genetic engineering perhaps exerts greatest influence in
biotech industry. A detailed knowledge of tools and
techniques in recombinant DNA and Genetic Engineering is an
absolute prerequisite for a successful career in
Biotechnology.
I. Fundamentals
1. Isolation of plant DNA SDS method,C-TAB method
2. Isolation of RNA
3. Isolation of animal DNA
4. Isolation of Plasmid DNA
5. Isolation of genomic DNA from prokaryotes
6. Quantitative and Qualitative analysis of DNA
II. Applied Techniques
1. Tm of DNA-Melting point of DNA
2. Agarose Gel Electrophoresis
3. Elusion of inserts from the Gel
4. Restriction Digestion
5. Ligation
6. Transformation
7. Gene cloning and expression
8. Southern Blotting
9. Western Blotting
10. PCR Techniques
11. DNA Finger printing(by RAPD)
Duration : 30 days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.E. (Biotek / Chemical)
Diploma in Enzymology
INTRODUCTION : Enzymes are biological catalysts,
regulating the entire life processes. A detailed knowledge
of enzyme Biotechnology is a prerequisite for success in
commercial Biotechnology, as enzyme occupy bulk share of
global biotech business. This course probides a detailed
insight into practical domain of Enzymes & Proteins.
1. Isolation of enzymes from bacterial and fungal source
2. Isolation of micro-organisms from soil by serial dilution
3. Pure culture preparation
4. Screening for the enzyme production
5. Batch Culture
a. Solid state fermentation
b. Submerged fermentation
6. Enzyme purification
a. Salt precipitation technique
b. Dialysis
c. Ion Exchange Chromatography
7. Enzyme assay
8. Quantitative estimation of Protein
9. Enzyme kinetic
a. Ph
b. Temperature
c. Substrate conc.
d. Activator
e. Inhibitor
10. SDS PAGE
11. Native PAGE (Theory)
12. Immobilization of Enzymes
Duration : 30 days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.E. (Biotek / Chemical)
Diploma in Fermentation and Downstream Processing
INTRODUCTION :
Fermentation and downstream processing is by far the most
important production systems in Biotech industry. Knowledge
of these provides the right perspective for individuals to
understand operation systems in Biotech industry
I. Fundamentals
1. Fermentation Fundamental
2. Submerged fermentation
3. Solid state fermentation
4. Fermentation systems
a. Conical flask
b. Vessels
c. Fermentors
II. Applied aspects depends on the desired products
(Citric acid,/Amino acids/ Enzymes/Antibiotics)
1. Down stream processing
a. Extra cellular
b. Intra cellular
2. Cell separation techniques
a. Centrifugation
b. Membrane filtration
c. Cell lysing
3. Isolation /Purification of desire products
a. Precipitation techniques
b. Solvent extraction
c. Dialysis
d. Ion exchange chromatography
e. Distillation
III. Analysis of Metabolites/Products
1. Thin layer chromatography
2. Melting point determination
3. Spectrophotometer determination
4. Titrometric
5. Electrophoresis
Duration : 30 Days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.E. (Biotek / Chemical)
Diploma in Immunological Techniques
INTRODUCTION :
Immunotechnology offers a comprehensive practical training
programme in immunodiagnostics and serological techniques.
This course provides in depth insight into world of
antibodies and diagnostics.
I. Agglutination and Precipitation Methods
1. Blood grouping and Rh typing
2. Fibril Agglutination test(Widal test)
3. VDRL Test/ RPR
4. Hbs Ag Test
II. Gel diffusion Methods
1. Ouchterlony Double Diffusion
2. Single Radial Immunodiffusion
3. Immunoelectrophoresis
4. Counter current immunoelectrophoresis
5. Rocket immunoelectrophoresis
III. Purification method
1. Isolation & Purification of Ig G from blood
sample
2. Salt Precipitation
3. Dialysis
4. Ion exchange Chromatography
5. Quantitative estimation of proteins
6. SDS PAGE
IV. Separation of lymphocytes
V. Raising polyclonal antibodies
VI. Immunochemistry method
1. ELISA
2. Pregnancy test
Duration : 30 Days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.Tech. B.E. (Biotek / Chemical)
Diploma in Plant Tissue
Culture
INTRODUCTION : Biotechnological advances in
agriculture have perhaps increased green revolution. From
hybrid seeds to edible vaccines, the impact of biotechnology
in agriculture is tremendous. This course provides an in
depth insight into world of modern plant biotechnology.
1. Instrumentation in Plant tissue culture
2. Sterilization technique:
3. Preparation of culture media:
i. Preparation of stock solutions of MS, B5, Nitsch and LS media
ii. Preparation of hormone stocks solution.
4. Callus culture:
i. Initiation and maintenance of callus from the inter nodal explants of
stem
explants of any available plant material
5. Embryo culture
6. Anther culture
7. Meristam culture
8. Root culture
9. Flower culture
10. Techniques involved in Agro bacterium mediated gene
transformation
a) Leaf Disc Preparation / callus preparation
b) Co-cultivation
c) Selection media transfer
11. Screening of transformed plants using PCR techniques
a. DNA isolation
b. PCR amplification
c. Agarose gel electrophoresis
d. Gel analysis
12. Encapsulation of embryo
Duration : 30 Days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.Tech. B.E. (Biotek / Chemical)
Diploma in Industrial Microbiology
INTRODUCTION :Micro-organisms are biological
factories, which provide majority of the biotechnological
products so far commercialized. With the advent of
recombinant DNA technology, the scope and importance of
microorganisms and its in depth study has assumed tremendous
importance. This course provides detailed enlightenment into
the practical world of microbial Biotechnology.
1. Sterility Techniques
2. Isolation of Micro-organisms from natural sources
a. Staining Technique
b. Grams staining
3. Endospore staining
4. Biochemical Tests
5. Bacterial Growth Curve
6. Mutation Studies
7. Screening of Micro Organisms for Industrial applications
a. Casein hydrolysis
b. Starch hydrolysis
c. Cellulose hydrolysis
8. Antibiotic assays
9. Quality control of milk
10. Water analysis
11. Sewage water treatment
12. Production and characterization of industrial
enzymes/wine/ citric acid
Duration : 30 Days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.Tech. B.E. (Biotek / Chemical)
Diploma in Medical
Biotechnology
INTRODUCTION :Medical Biotechnology is the
study of the causes and management of infectious diseases.
These can be caused by viruses, bacteria, fungi and
protozoa. By the end of this course students should be able
to demonstrate advanced knowledge and understanding of the
nature of viruses, bacteria, parasites and fungi and basic
criteria used in theclassification/taxonomy of these
micro-organisms;
I. Fundamentals
1. Isolation of pathogens from clinical sample
2. Identification(Preliminary) of pathogens
3. Biochemical confirmation of the isolate
4. Any special test (if relevant)
5. Antibiotic sensitivity test
6. Review of analysis
7. Report preparation
II. Applied Techniques
1. Imunological Techniques
a. Precipitation and agglutination tests
i. Related to the diagnosis of pathogens
(CRP, RPR, VDRL Widal SOPS)
2. Biochemical tests
a. Detection of blood group and Rh factor
b. Haemoglobulin test
c. Pregnancy test
d. Detection of urinary protein
e. Detection of urinary glucose
f. Detection of ketone bodies in urine sample
3. WBC count
4. MIC and MLC
5. Introduction to Bioinformatics.
Duration : 30 Days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.Tech. B.E. (Biotek / Chemical) |
