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Introduction:
Biotechnology is multi-disciplinary science comprising
various facets of biology. This course provides an overall
view of practical aspects of biotech industry.
I Industrial microbiology
1. Sterility Techniques
2. Isolation of Micro-organisms from natural sources
3. Staining Technique
i) Grams staining
ii) Endospore staining
4. Biochemical Tests
i) Gelatin liquification
ii) Catalase test
iii) TSI test
5. Bacterial Growth Curve
6. Mutation Studies
7. Screening of Micro Organisms for Industrial applications
i) Caesein hydrolysis
ii) Starch hydrolysis
iii) Cellulose hydrolysis
8. Antibiotic assays
9. Quality control of milk
10. Water analysis
II Enzymology
1. Isolation of micro-organisms from soil by serial
dilution
2. Pure culture preparation
3. Screening for the enzyme production
4. Batch Culture
a. Solid state fermentation
b. Submerged fermentation
5. Enzyme purification
a. Salt precipitation technique
b. Dialysis
6. Ion Exchange Chromatography
7. Enzyme assay
8. Quantitative estimation of Protein
9. Enzyme kinetic
a. Ph
b. Temperature
c. Substrate conc.
d. Activator
e. Inhibitor
10. SDS PAGE
11. Immobilization of Enzymes
III RECOMBINANT DNA TECHNOLOGY
Fundamentals
1. Isolation of plant DNA SDS method,C-TAB method
2. Isolation of RNA
3. Isolation of animal DNA
4. Isolation of Plasmid DNA
5. Isolation of genomic DNA from prokaryotes
6. Quantitative and Qualitative analysis of DNA
Applied Techniques
1. Tm of DNA-Melting point of DNA
2. Agarose Gel Electrophoresis
3. Elusion of inserts from the Gel
4. Restriction Digestion
5. Ligation
6. Transformation
7. Gene cloning and expression
8. Southern Blotting
9. Western Blotting
10. PCR Techniques
11. DNA Finger printing(by RAPD)
IV. BIO-PHYSICALCHEMISTRY
HPLC-Analysis
All Aspects of basic Programme plus independently
operating the HPLC system and allied equipment as well as
independently performing various operation in HPLC analysis
including preparation of mobile phase, cleaning of
pre-filter/injecting the samples/standard solutions,
recording, storage and retrieval of analysis.
DURATION : 60 Days
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.E. (Biotek / Chemical) |
