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Techniques Involved in RAPD
Analysis
1. Plant DNA Isolation
2. Purification of Isolated DNA
3. PCR amplification
4. Agarose gel elctrophoresis
5. Gel analysis / gel documentation
Duration : 7 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, P.hd., B.Tech. B.E. (Chemical / Biotech)
HPLC Analysis
Fundamental Programme :All aspects of Basic
Programme plus independently operating the HPLC system and
allied equipment as well as independently performing various
operation in HPLC analysis including preparation of mobile
phase, cleaning of pre-filter/injector, attaching column,
preparation of solution for analysis, injecting the
samples/standard solutions, recording, storage andretrieval
of analysis data etc,.
Duration : 7 Days
Eligibility :M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, P.hd., B.Tech. B.E. (Chemical / Biotech)
Gel Electrophoresis Techniques
1. Agarose gel electrophoresis
2. SDS- Poly Acralamide gel electrophoresis
3. NATIVE- Poly Acralamide gel electrophoresis
4. Immuno electrophoresis
Duration : 7 Days
Eligibility :M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, P.hd., B.Tech. B.E. (Chemical / Biotech)
Chromatographic Techniques
1. Paper chromatography
2. Thin Layer chromatography
3. Column Chromatography
a. Ion exchange chromatography
b. Affinity chromatography
c. Gel filtration chromatography
d. High performance liquid chromatography
Duration : 10 Days
Eligibility :M.Sc. / B.Sc. (Life Science), M.Pharma /
B.Pharma M.Phil, P.hd., B.Tech. B.E. (Chemical / Biotech)
Screening of transgenic plants using PCR
1. DNA Isolation of transgenic plant
2. Purification of DNA
3. PCR amplification
4. Agarose gel electrophoresis
5. Gel documentation
Duration : 10 Days
Eligibility : M.Sc. / B.Sc. (Life Science), M.Pharma
/ B.Pharma M.Phil, P.hd., B.Tech. B.E. (Chemical / Biotech) |
