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INTRODUCTION : Biotechnology is a multi-disciplinary
science comprising of various facets of Biology. This course
provides advanced practical training aspect of Biotech
industry.
MODULE I
Microbial Biotechnology
1. Fundamentals in Microbiology
2. Sterilization Techniques
3. Microbiological culture media and its importance in
Biotechnology
4. Isolation of Micro-organisms from air and soil
5. Pure culture preparation
6. Identification of organisms by
a. Staining techniques
b. Biochemical tests
7. Growth curve
Applied Techniques
1. Antibiotic assays
2. Mutational studies by Replica plating method
3. Bacterial conjugation
4. Screening for Enzymes
5. Antogonism studies
6. Isolation of protozoa from samples
7. Isolation & Identification of fungi
8. Production of secondary metabolites
(Acids, Wine, Enzymes, Antibiotics)
MODULE II
Recombinant DNA Technology
Fundamentals
1. Isolation of plant DNA SDS method,C-TAB method
2. Isolation of RNA
3. Isolation of animal DNA
4. Isolation of Plasmid DNA
5. Isolation of genomic DNA from prokaryotes
6. Quantitative and Qualitative analysis of DNA
Applied Techniques
1. Tm of DNA-Melting point of DNA
2. Agarose Gel Electrophoresis
3. Elusion of inserts from the Gel
4. Restriction Digestion
5. Ligation
6. Transformation
7. Gene cloning and expression
8. Southern Blotting
9. Western Blotting
10. PCR Techniques
MODULE III
Enzymology
1. Isolation of enzymes from bacterial and fungal
source
2. Isolation of micro-organisms from soil by serial dilution
3. Pure culture preparation
4. Screening for the enzyme production
5. Batch Culture
a. Solid state fermentation
b. Submerged fermentation
6. Enzyme purification
a. Salt precipitation technique
b. Dialysis
c. Ion Exchange Chromatography
7. Enzyme assay
8. Quantitative estimation of Protein
9. Enzyme kinetic
a. Ph
b. Temperature
c. Substrate conc.
d. Activator
e. Inhibitor
10. SDS PAGE
11. Native PAGE (Theory)
12. Immobilization of Enzymes
MODULE IV Immunological Techniques
A. Agglutination and Precipitation Methods
1. Blood grouping and Rh typing
2. Fibril Agglutination test(Widal test)
3. VDRL Test/ RPR
4. Hbs Ag Test
B. Gel diffusion Methods
1. Ouchterlony Double Diffusion
2. Single Radial Immunodiffusion
3. Immunoelectrophoresis
4. Counter current immunoelectrophoresis
5. Rocket immunoelectrophoresis
C. Purification method
1. Isolation & Purification of Ig G from blood sample
2. Salt Precipitation
3. Dialysis
4. Ion exchange Chromatography
5. Quantitative estimation of proteins
6. SDS PAGE
D. Separation of lymphocytes
E. Raising polyclonal antibodies
F. Immunochemistry method
2. ELISA
3. Pregnancy test
MODULE V - Bio-Physical Chemistry
HPLC-Analysis
All Aspects of basic Programme plus independently
operating the HPLC system and allied equipment as well as
independently
performing various operation in HPLC analysis including
preparation of mobile phase, cleaning of
pre-filter/injecting the
samples/standard solutions, recording, storage and retrieval
of analysis
MODULE VI
Project dissertation
MODULE VII
Industrial visit
Eligibility : B.sc. / M.Sc. (all Streams of Life
Science) M.Phil., B.E. (Biotek / Chemical) |
